Can we build a better heart outside the body? This innovative research explores the creation of engineered heart tissue (EHT) exhibiting key characteristics of differentiated myocardium. Cardiac myocytes from neonatal rats are combined with collagen I and matrix factors, molded into rings, and subjected to mechanical stretch, resulting in ring-shaped EHTs. Comparative histological analysis reveals that the cardiac cells in EHTs form interconnected muscle bundles resembling adult heart tissue. Confocal and electron microscopy confirm the presence of highly organized sarcomeres and other features of differentiated myocardium. EHTs demonstrate contractile characteristics and action potential recordings similar to native myocardium. The results suggest EHTs represent a promising material for in vitro studies of cardiac function and potential tissue replacement therapy. This work highlights the potential of cardiac tissue engineering to revolutionize cardiac research and treatment.
Published in _Circulation Research_, a leading journal in cardiovascular science, this article highlights cutting-edge research in cardiac tissue engineering. The study's focus on creating functional cardiac muscle constructs aligns perfectly with the journal's mission to advance understanding and treatment of heart disease.