Can surface plasmon resonance imaging revolutionize biomolecular studies? This review explores the use of surface plasmon resonance (SPR) imaging to characterize ultrathin organic and biopolymer films at metal interfaces. The technique's high surface sensitivity and real-time measurement capabilities for unlabeled biological molecules make it a promising tool for biomolecular investigations. The review details SPR imaging's principles, instrumental design, and capabilities, such as monitoring antibody-antigen interactions and studying DNA hybridization adsorption. The technique involves the fabrication of DNA arrays on chemically modified gold surfaces and monitoring protein DNA interactions. SPR imaging has the potential to significantly impact various fields by enabling real-time monitoring of biomolecular interactions. This technique is particularly valuable for applications in drug discovery, diagnostics, and biosensing, paving the way for advancements in personalized medicine and biotechnology. Furthermore, the ability to spatially resolve ultrathin films and interfaces offers new avenues for materials science research and development.
Published in the Annual Review of Physical Chemistry, this article provides a comprehensive overview of SPR imaging, a technique used in the study of ultrathin films and biomolecular interactions. The article aligns with the journal's scope by presenting the principles, instrumentation, and applications of SPR imaging, thus contributing to advancements in physical chemistry and related fields.
| Category | Category Repetition |
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| Science: Chemistry | 236 |
| Technology: Chemical technology | 111 |
| Science: Chemistry: Analytical chemistry | 110 |
| Science: Physics | 95 |
| Science: Chemistry: Physical and theoretical chemistry | 93 |