Titel	Veröffentlichungsdatum	Sprache	Zitate
An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol	2008/01/01	English	720
Mechanism of chimera formation during the Multiple Displacement Amplification reaction	2007/01/01		197
A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering	2010/01/01	English	167
A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR	2008/01/01	English	149
Gene knockdown by RNAi in the pea aphid Acyrthosiphon pisum	2007/01/01		139
Improved Tet-responsive promoters with minimized background expression	2010/01/01	English	139
Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering	2007/01/01		129
Isolation of mesenchymal stem cells from equine umbilical cord blood	2007/01/01		123
A suite of recombinant luminescent bacterial strains for the quantification of bioavailable heavy metals and toxicity testing	2009/01/01	English	122
Measuring microRNAs: Comparisons of microarray and quantitative PCR measurements, and of different total RNA prep methods	2008/01/01	English	111
One-step selection of Vaccinia virus-binding DNA aptamers by MonoLEX	2007/01/01		108
High-efficiency protein expression in plants from agroinfection-compatible Tobacco mosaic virus expression vectors	2007/01/01		103
Self-assembling Fmoc dipeptide hydrogel for in situ 3D cell culturing	2007/01/01	English	93
Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences	2009/01/01	English	83
Potential pitfalls in the accuracy of analysis of natural sense-antisense RNA pairs by reverse transcription-PCR	2007/01/01		79
Comparison of estimation capabilities of response surface methodology (RSM) with artificial neural network (ANN) in lipase-catalyzed synthesis of palm-based wax ester	2007/01/01	English	65
Systolic and diastolic ventricular function in zebrafish embryos: Influence of norepenephrine, MS-222 and temperature	2008/01/01	English	65
Collagen matrices from sponge to nano: new perspectives for tissue engineering of skeletal muscle	2009/01/01	English	64
Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR	2009/01/01	English	64
Characterization of the effect of sample quality on high density oligonucleotide microarray data using progressively degraded rat liver RNA	2007/01/01		60
Cell-free protein synthesis energized by slowly-metabolized maltodextrin	2009/01/01	English	59
Optimization of codon composition and regulatory elements for expression of human insulin like growth factor-1 in transgenic chloroplasts and evaluation of structural identity and function	2009/01/01	English	56
Simplified production and concentration of HIV-1-based lentiviral vectors using HYPERFlask vessels and anion exchange membrane chromatography	2009/01/01	English	51
Differential protein expression following low temperature culture of suspension CHO-K1 cells	2008/01/01	English	50
High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis	2008/01/01	English	47
Laser-scanning velocimetry: A confocal microscopy method for quantitative measurement of cardiovascular performance in zebrafish embryos and larvae	2007/01/01		46
Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers	2008/01/01	English	45
Plasmid selection in Escherichia coli using an endogenous essential gene marker	2008/01/01	English	45
Dual-In/Out strategy for genes integration into bacterial chromosome: a novel approach to step-by-step construction of plasmid-less marker-less recombinant E. coli strains with predesigned genome structure	2008/01/01	English	42
Impact of point-mutations on the hybridization affinity of surface-bound DNA/DNA and RNA/DNA oligonucleotide-duplexes: Comparison of single base mismatches and base bulges	2008/01/01	English	40