Title	Publication Date	Language	Citations
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs	1997/09/01		51,834
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools	1997/12/15		30,178
MUSCLE: multiple sequence alignment with high accuracy and high throughput	2004/03/08	English	28,683
A new mathematical model for relative quantification in real-time RT-PCR	2001/05/01		25,185
The Protein Data Bank	2000/01/01		24,584
KEGG: Kyoto Encyclopedia of Genes and Genomes	2000/01/01		20,462
limma powers differential expression analyses for RNA-sequencing and microarray studies	2015/01/20	English	19,844
The SILVA ribosomal RNA gene database project: improved data processing and web-based tools	2012/11/27	English	17,159
Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists	2008/11/25	English	10,398
MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform	2002/07/15		10,090
STRING v11: protein–protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets	2018/11/22	English	9,815
Mfold web server for nucleic acid folding and hybridization prediction	2003/07/01	English	9,723
ANNOVAR: functional annotation of genetic variants from high-throughput sequencing data	2010/07/03	English	8,870
Gene Expression Omnibus: NCBI gene expression and hybridization array data repository	2002/01/01		8,559
SWISS-MODEL: homology modelling of protein structures and complexes	2018/05/21	English	7,076
STRING v10: protein–protein interaction networks, integrated over the tree of life	2014/10/28	English	6,786
MEME SUITE: tools for motif discovery and searching	2009/05/20	English	6,533
Primer3—new capabilities and interfaces	2012/06/21	English	6,211
Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCR	2002/05/01		5,650
GEPIA: a web server for cancer and normal gene expression profiling and interactive analyses	2017/04/12	English	5,644
Loop-mediated isothermal amplification of DNA	2000/06/15		5,640
Tandem repeats finder: a program to analyze DNA sequences	1999/01/01	English	5,407
NCBI GEO: archive for functional genomics data sets—update	2012/11/26	English	5,260
Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies	2012/08/28	English	5,224
Enrichr: a comprehensive gene set enrichment analysis web server 2016 update	2016/05/03	English	5,215
tRNAscan-SE: A Program for Improved Detection of Transfer RNA Genes in Genomic Sequence	1997/03/01	English	5,212
KEGG: new perspectives on genomes, pathways, diseases and drugs	2016/11/28	English	4,988
The PRIDE database and related tools and resources in 2019: improving support for quantification data	2018/11/05	English	4,965
dbSNP: the NCBI database of genetic variation	2001/01/01		4,717
SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB	2007/11/14	English	4,692