Analytical Biochemistry

Title Publication Date Language Citations
Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: Results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes1980/11/01English820
A rapid method for the base ratio determination of bacterial DNA1977/08/01English815
A Self-Consistent Method for the Analysis of Protein Secondary Structure from Circular Dichroism1993/02/01English815
Quantitative monitoring of solid-phase peptide synthesis by the ninhydrin reaction1981/10/01English807
Circular dichroic analysis of protein conformation: Inclusion of the β-turns1978/11/01English803
Measurement of catalase activity in tissue extracts1970/03/01English795
Ellman's reagent: 5,5′-dithiobis(2-nitrobenzoic acid)—a reexamination1979/04/01English789
A Fluorometric Assay for the Measurement of Nitrite in Biological Samples1993/10/01English782
An improved staining procedure for the detection of the peroxidase activity of cytochrome P-450 on sodium dodecyl sulfate polyacrylamide gels1976/09/01English775
Quantitative Reverse Transcription–Polymerase Chain Reaction to Study mRNA Decay: Comparison of Endpoint and Real-Time Methods2000/10/01English771
A direct colorimetric assay for Ca2+-stimulated ATPase activity1986/09/01English759
Synthesis of a Fluorescent Derivatizing Reagent, 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate, and Its Application for the Analysis of Hydrolysate Amino Acids via High-Performance Liquid Chromatography1993/06/01English756
Extraction and Determination of Ascorbate and Dehydroascorbate from Plant Tissue1995/02/01English754
Linearization of the Bradford Protein Assay Increases Its Sensitivity: Theoretical and Experimental Studies1996/05/01English752
A Modified Hot Borate Method Significantly Enhances the Yield of High-Quality RNA from Cotton (Gossypium hirsutum L.)1994/11/01English750
Modifications of a specific assay for hydroxyproline in urine1967/05/01English745
Enthalpy of decomposition of hydrogen peroxide by catalase at 25° C (with molar extinction coefficients of H2O2 solutions in the UV)1972/10/01English740
Review of the folin phenol protein quantitation method of lowry, rosebrough, farr and randall1979/12/01English733
A specific method for the analysis of hydroxyproline in tissues and urine1960/11/01English725
Simultaneous determination of hemes a, b, and c from pyridine hemochrome spectra1987/02/01English718
A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels1979/09/01English711
A spectrophotometric method for determination of catalase activity in small tissue samples1988/10/01English708
A simple technique for eliminating interference by detergents in the Lowry method of protein determination1975/03/01English702
Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea1986/05/01English691
Solubilization and Purification of Enzymatically Active Glutathione S-Transferase (pGEX) Fusion Proteins1993/04/01English690
A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphatase1981/05/01English676
Reevaluation of assay methods and establishment of kit for superoxide dismutase activity1984/11/01English669
Assay method for myeloperoxidase in human polymorphonuclear leukocytes1983/07/01English667
Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions1986/07/01English655
The avidin-biotin complex in bioanalytical applications1988/05/01English649