Title	Publication Date	Language	Citations
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors	1985/01/01	English	11,036
Site-directed mutagenesis by overlap extension using the polymerase chain reaction	1989/04/01	English	5,751
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers	1982/10/01	English	4,108
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase	1988/07/01	English	4,041
Construction and characterization of new cloning vehicle. II. A multipurpose cloning system	1977/11/01	English	3,577
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing	1984/06/01	English	2,839
A simple and very efficient method for generating cDNA libraries	1983/11/01	English	2,753
Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes	1995/12/01	English	2,704
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension	1989/04/01	English	2,306
Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum	1994/07/01	English	2,214
New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites	1988/12/01	English	2,148
The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions	1988/11/01	English	2,148
CLUSTAL: a package for performing multiple sequence alignment on a microcomputer	1988/12/01	English	2,124
FACS-optimized mutants of the green fluorescent protein (GFP)	1996/01/01	English	2,021
A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformaion of Escherichia coli	1987/01/01	English	1,781
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments	1982/10/01	English	1,748
Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis	1983/12/01	English	1,638
Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds	1995/04/01	English	1,485
The ‘effective number of codons’ used in a gene	1990/03/01	English	1,404
Epidermal growth factor receptor (EGFR) signaling in cancer	2006/01/01	English	1,347
Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria	1988/10/01	English	1,208
In vitro insertional mutagenesis with a selectable DNA fragment	1984/09/01	English	1,172
Acetylation and deacetylation of non-histone proteins	2005/12/01	English	1,155
Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.	1992/07/01	English	1,136
Improved single and multicopy lac-based cloning vectors for protein and operon fusions	1987/01/01	English	1,121
Genetics and biology of vitamin D receptor polymorphisms	2004/09/01	English	1,023
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells	1979/01/01	English	914
Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector	1986/01/01	English	815
Specific-purpose plasmid cloning vectors II. Broad host range, high copy number, RSF 1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas	1981/12/01	English	793
Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli	1988/09/01	English	786