Need a faster way to detect RNA? This review explores PCR-free techniques as an alternative to traditional RT-qPCR for RNA detection. It highlights the limitations of RT-qPCR, including its complexity, high cost, and reliance on professional technicians. The article reviews current PCR-free methods, providing an overview of reported RNA biosensors based on electrochemistry, SPR, microfluidics, nanomaterials, and CRISPR. It highlights PCR-free techniques as low-cost, simple operation methods that do not require large instruments. The study discusses the challenges and future research prospects in RNA detection. This review offers a new concept for the development of new RNA detection methods, with potential applications in disease diagnosis, medical research, and genetic engineering. PCR-free techniques are attractive due to their simplicity and low cost. Future research should focus on improving the sensitivity and specificity of these methods to enable their widespread adoption.
Published in 10.3390/bios14040, this review aligns with the journal's focus on biosensors and bioengineering. It provides insights into alternative techniques for RNA detection. The review has relevance to biotechnology, analytical chemistry, and instrumentation, emphasizing analytical methodologies and recent advancements in biosensors.
| Category | Category Repetition |
|---|---|
| Science: Chemistry: Analytical chemistry | 87 |
| Science: Chemistry | 51 |
| Science: Biology (General) | 23 |
| Technology: Chemical technology | 22 |
| Science: Chemistry: Organic chemistry: Biochemistry | 21 |