Activation Tagging in Arabidopsis

Article Properties
  • Language
    English
  • Publication Date
    2000/04/01
  • Indian UGC (Journal)
  • Refrences
    60
  • Citations
    627
  • Detlef Weigel Plant Biology Laboratory (D.W., J.H.A., M.A.B., J.O.B., S.K.C., C.Fankhauser, I.K., E.J.M., M.M.N., J.T.N., Z.-Y.W., Y.X., C.J.L., J.C.) and
  • Ji Hoon Ahn Plant Biology Laboratory (D.W., J.H.A., M.A.B., J.O.B., S.K.C., C.Fankhauser, I.K., E.J.M., M.M.N., J.T.N., Z.-Y.W., Y.X., C.J.L., J.C.) and
  • Miguel A. Blázquez Plant Biology Laboratory (D.W., J.H.A., M.A.B., J.O.B., S.K.C., C.Fankhauser, I.K., E.J.M., M.M.N., J.T.N., Z.-Y.W., Y.X., C.J.L., J.C.) and
  • Justin O. Borevitz Plant Biology Laboratory (D.W., J.H.A., M.A.B., J.O.B., S.K.C., C.Fankhauser, I.K., E.J.M., M.M.N., J.T.N., Z.-Y.W., Y.X., C.J.L., J.C.) and
  • Sioux K. Christensen Plant Biology Laboratory (D.W., J.H.A., M.A.B., J.O.B., S.K.C., C.Fankhauser, I.K., E.J.M., M.M.N., J.T.N., Z.-Y.W., Y.X., C.J.L., J.C.) and
  • Christian Fankhauser Plant Biology Laboratory (D.W., J.H.A., M.A.B., J.O.B., S.K.C., C.Fankhauser, I.K., E.J.M., M.M.N., J.T.N., Z.-Y.W., Y.X., C.J.L., J.C.) and
  • Cristina Ferrándiz Department of Biology, University of California at San Diego, La Jolla, California 92093–0116 (C.Ferrándiz, S.S., M.F.Y.); and
  • Igor Kardailsky Plant Biology Laboratory (D.W., J.H.A., M.A.B., J.O.B., S.K.C., C.Fankhauser, I.K., E.J.M., M.M.N., J.T.N., Z.-Y.W., Y.X., C.J.L., J.C.) andPlant Biology Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, Oklahoma 73402 (I.K., Y.X., R.A.D., M.J.H.)
  • Elizabeth J. Malancharuvil Plant Biology Laboratory (D.W., J.H.A., M.A.B., J.O.B., S.K.C., C.Fankhauser, I.K., E.J.M., M.M.N., J.T.N., Z.-Y.W., Y.X., C.J.L., J.C.) and
  • Michael M. Neff Plant Biology Laboratory (D.W., J.H.A., M.A.B., J.O.B., S.K.C., C.Fankhauser, I.K., E.J.M., M.M.N., J.T.N., Z.-Y.W., Y.X., C.J.L., J.C.) and
  • Jasmine Thuy Nguyen Plant Biology Laboratory (D.W., J.H.A., M.A.B., J.O.B., S.K.C., C.Fankhauser, I.K., E.J.M., M.M.N., J.T.N., Z.-Y.W., Y.X., C.J.L., J.C.) and
  • Shusei Sato Department of Biology, University of California at San Diego, La Jolla, California 92093–0116 (C.Ferrándiz, S.S., M.F.Y.); and
  • Zhi-Yong Wang Plant Biology Laboratory (D.W., J.H.A., M.A.B., J.O.B., S.K.C., C.Fankhauser, I.K., E.J.M., M.M.N., J.T.N., Z.-Y.W., Y.X., C.J.L., J.C.) and
  • Yiji Xia Plant Biology Laboratory (D.W., J.H.A., M.A.B., J.O.B., S.K.C., C.Fankhauser, I.K., E.J.M., M.M.N., J.T.N., Z.-Y.W., Y.X., C.J.L., J.C.) andPlant Biology Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, Oklahoma 73402 (I.K., Y.X., R.A.D., M.J.H.)
  • Richard A. Dixon Plant Biology Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, Oklahoma 73402 (I.K., Y.X., R.A.D., M.J.H.)
  • Maria J. Harrison Plant Biology Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, Oklahoma 73402 (I.K., Y.X., R.A.D., M.J.H.)
  • Chris J. Lamb Plant Biology Laboratory (D.W., J.H.A., M.A.B., J.O.B., S.K.C., C.Fankhauser, I.K., E.J.M., M.M.N., J.T.N., Z.-Y.W., Y.X., C.J.L., J.C.) and
  • Martin F. Yanofsky Department of Biology, University of California at San Diego, La Jolla, California 92093–0116 (C.Ferrándiz, S.S., M.F.Y.); and
  • Joanne Chory Plant Biology Laboratory (D.W., J.H.A., M.A.B., J.O.B., S.K.C., C.Fankhauser, I.K., E.J.M., M.M.N., J.T.N., Z.-Y.W., Y.X., C.J.L., J.C.) andHoward Hughes Medical Institute (J.C.), The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037;
Abstract
Cite
Weigel, Detlef, et al. “Activation Tagging in Arabidopsis”. Plant Physiology, vol. 122, no. 4, 2000, pp. 1003-14, https://doi.org/10.1104/pp.122.4.1003.
Weigel, D., Ahn, J. H., Blázquez M. A., Borevitz, J. O., Christensen, S. K., Fankhauser, C., Ferrándiz C., Kardailsky, I., Malancharuvil, E. J., Neff, M. M., Nguyen, J. T., Sato, S., Wang, Z.-Y., Xia, Y., Dixon, R. A., Harrison, M. J., Lamb, C. J., Yanofsky, M. F., & Chory, J. (2000). Activation Tagging in Arabidopsis. Plant Physiology, 122(4), 1003-1014. https://doi.org/10.1104/pp.122.4.1003
Weigel D, Ahn JH, Blázquez MA, Borevitz JO, Christensen SK, Fankhauser C, et al. Activation Tagging in Arabidopsis. Plant Physiology. 2000;122(4):1003-14.
Journal Categories
Agriculture
Animal culture
Agriculture
Plant culture
Science
Botany
Science
Botany
Plant ecology
Science
Zoology
Description

Unlock the potential of plant genes: This study introduces activation tagging as a powerful tool for gene discovery in *Arabidopsis*. By using T-DNA vectors containing multimerized transcriptional enhancers from the cauliflower mosaic virus (CaMV) 35S gene, researchers generated thousands of transformed plants and identified over 30 dominant mutants with diverse phenotypes. Analysis revealed that overexpressed genes are typically located near the CaMV 35S enhancers, suggesting a direct activation mechanism. Interestingly, in some cases, the enhancers primarily enhanced the endogenous expression pattern rather than causing ectopic expression. This method provides a valuable approach for identifying genes involved in various plant processes and understanding the regulatory mechanisms that control gene expression. The findings have important implications for plant biotechnology and crop improvement.

Published in Plant Physiology, this paper is well-suited to the journal's focus on plant biology and biochemistry. By describing a novel method for gene discovery in Arabidopsis, the study provides valuable tools and insights for plant researchers. The findings on activation tagging and its effects on gene expression contribute to the understanding of fundamental processes in plant development and physiology.

Refrences
Refrences Analysis
The category Science: Biology (General) 25 is the most frequently represented among the references in this article. It primarily includes studies from Nature and Cell. The chart below illustrates the number of referenced publications per year.
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Citations
Citations Analysis
The first research to cite this article was titled RSF1, an Arabidopsis Locus Implicated in Phytochrome A Signaling and was published in 2000. The most recent citation comes from a 2024 study titled RSF1, an Arabidopsis Locus Implicated in Phytochrome A Signaling . This article reached its peak citation in 2006 , with 52 citations.It has been cited in 164 different journals, 15% of which are open access. Among related journals, the The Plant Journal cited this research the most, with 75 citations. The chart below illustrates the annual citation trends for this article.
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