Boosting RNA cleavage with multivalent DNAzymes: This study explores multivalent recognition and binding to enhance the efficiency and specificity of RNA cleavage by DNAzymes (Dz). Inspired by natural multivalent interactions, the researchers designed bivalent Dz devices (BDD) containing two Dz agents. One BDD increased the cleavage efficiency of a folded RNA fragment up to 17-fold compared to conventional Dz designs. This enhancement resulted from improved RNA binding and increased cleavage probability by the two catalytic cores. By controlling Dz agent association in BDD, excellent selectivity was achieved in differentiating single-base mismatched RNA while maintaining high cleavage rates. Furthermore, a trivalent Dz showed even greater efficiency. The data suggests that cooperative action of multiple RNA-cleaving units can significantly improve RNA cleavage, which is important for developing Dz-based gene knockdown agents.
Published in Nucleic Acids Research, this article aligns perfectly with the journal's focus on cutting-edge research in nucleic acids. The study's exploration of multivalent DNAzymes for RNA cleavage falls directly within the journal's scope, which emphasizes advancements in understanding and manipulating nucleic acid structures and functions.
| Category | Category Repetition |
|---|---|
| Science: Biology (General) | 37 |
| Science: Chemistry: Organic chemistry: Biochemistry | 24 |
| Science: Biology (General): Genetics | 11 |
| Medicine: Medicine (General) | 10 |
| Science: Chemistry | 7 |