Biomolecular Detection and Quantification

Title Publication Date Language Citations
How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments2015/03/01English340
Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR)2017/06/01English325
The emerging role of cell-free DNA as a molecular marker for cancer management2019/03/01English310
Assessing the performance of the Oxford Nanopore Technologies MinION2015/03/01English297
qPCR primer design revisited2017/12/01English146
Fundamentals of multiplexing with digital PCR2016/12/01English141
Determining lower limits of detection of digital PCR assays for cancer-related gene mutations2014/09/01English119
A survey of tools for the analysis of quantitative PCR (qPCR) data2014/09/01English107
Digital PCR: A brief history2014/09/01English104
Three-color crystal digital PCR2016/12/01English96
Proximity assays for sensitive quantification of proteins2015/06/01English74
Removal of between-run variation in a multi-plate qPCR experiment2015/09/01English72
Amplification of nonspecific products in quantitative polymerase chain reactions (qPCR)2017/12/01English72
Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms2016/03/01English65
Considerations and quality controls when analyzing cell-free tumor DNA2019/03/01English61
Multi-template polymerase chain reaction2014/12/01English58
Small sample sizes in high-throughput miRNA screens: A common pitfall for the identification of miRNA biomarkers2018/05/01English58
Validation of a digital PCR method for quantification of DNA copy number concentrations by using a certified reference material2016/09/01English47
Next-generation sequencing applications in clinical bacteriology2017/12/01English47
Quantification of mitochondrial DNA copy number in suspected cancer patients by a well optimized ddPCR method2017/09/01English46
Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing—Enabling robust and non-invasive biomarker research2019/03/01English42
A current overview of commercially available nucleic acid diagnostics approaches to detect and identify human gastroenteritis pathogens2014/09/01English41
The reproducibility of biomedical research: Sleepers awake!2014/12/01English38
Digital PCR dynamic range is approaching that of real-time quantitative PCR2016/12/01English38
The Unyvero P55 ‘sample-in, answer-out’ pneumonia assay: A performance evaluation2017/09/01English37
Effects of post-mortem and physical degradation on RNA integrity and quality2015/09/01English36
An optimized targeted Next-Generation Sequencing approach for sensitive detection of single nucleotide variants2018/05/01English32
Molecular techniques for the personalised management of patients with chronic myeloid leukaemia2017/03/01English32
Heparinase treatment of heparin-contaminated plasma from coronary artery bypass grafting patients enables reliable quantification of microRNAs2016/06/01English30
How to speed up the polymerase chain reaction2017/06/01English25